The Interface of Gene Editing with Regenerative Medicine

Area of Science:

• Regenerative Medicine
• Genome Engineering
• Gene Editing Technologies

Background:

• Regenerative medicine aims to repair tissues and restore function, with existing therapies approved for wound healing and orthopedic injuries.
• Current strategies utilize progenitor cells and biological stimuli but face challenges like off-target effects and lack of precision.
• The field is evolving towards more precise and efficient genome engineering technologies.

Purpose of the Study:

• To review the progress and future prospects of CRISPR technologies in regenerative medicine.
• To explore how gene editing advances therapeutic applications and aids research in tissue development and disease progression.

Main Methods:

• Review of CRISPR-based gene editing systems.
• Analysis of CRISPR applications in regenerative medicine and tissue engineering.
• Exploration of CRISPR's role as a research tool.

Main Results:

• CRISPR enables precise gene editing, including knock-ins, knockouts, transcriptional modulation, and base conversions.
• CRISPR facilitates the treatment of genetic and degenerative diseases.
• CRISPR aids in controlling cell fate for tissue repair and enhancing tissue function.

Conclusions:

• CRISPR technologies represent a significant advancement in regenerative medicine, offering precise and versatile tools.
• Gene editing with CRISPR is transforming therapeutic applications and deepening our understanding of biological processes.
• The integration of CRISPR promises to overcome limitations of traditional regenerative medicine approaches.