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Updated: Sep 9, 2025

Mitochondrial Respiration Quantification in Yeast Whole Cells
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Probing Intracellular Yeast Metabolism With Deuterium Magnetic Resonance Spectroscopy.

Fatima Anum1, Charbel Assaf1, Farhad Haj Mohamad1

  • 1Section Biomedical Imaging, Molecular Imaging North Competence Center, Department of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Kiel University, Kiel, Germany.

NMR in Biomedicine
|September 2, 2025
PubMed
Summary
This summary is machine-generated.

Deuterium magnetic resonance spectroscopy (DMRS) offers a noninvasive method to track metabolic flux in real-time, overcoming limitations of traditional NMR. This technique successfully monitored nutrient consumption and metabolic changes in yeast cells, demonstrating its versatility.

Keywords:
Saccharomyces cerevisiaeDMRSdeuterium labelingintracellular metabolismmetabolomicsnoninvasiveosmotic stressreal‐time flux measurement

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Area of Science:

  • Metabolomics
  • Biochemistry
  • Spectroscopy

Background:

  • Traditional 1H NMR struggles with signal overlap in complex biological samples like cells and tissues.
  • Metabolomics aims to capture metabolic states noninvasively, but limitations exist for intact biological systems.
  • Nuclear magnetic resonance (NMR) is a key noninvasive technique in metabolomics.

Purpose of the Study:

  • To introduce and validate Deuterium Magnetic Resonance Spectroscopy (DMRS) as a method for metabolic flux analysis.
  • To demonstrate DMRS's capability in tracking real-time metabolite consumption and metabolic pathway alterations.
  • To assess DMRS parameters for optimizing measurements in biological systems.

Main Methods:

  • Utilized deuterium-labeled tracers to circumvent background interference in NMR spectroscopy.
  • Measured key spectroscopic parameters (T1, T2, chemical shifts) for 26 deuterated compounds.
  • Applied DMRS to Saccharomyces cerevisiae (baker's yeast) under varied substrate concentrations and osmotic pressures.

Main Results:

  • Successfully tracked the real-time consumption of pyruvate, glucose, fumarate, acetone, and nicotinamide in yeast.
  • Demonstrated the ability to redirect yeast metabolism by manipulating pyruvate levels and buffer density.
  • Established optimal DMRS settings through characterization of deuterated compounds.

Conclusions:

  • DMRS is a robust and versatile tool for dissecting metabolic transformations noninvasively.
  • The study highlights DMRS's potential for real-time metabolic flux analysis in accessible model systems like yeast.
  • DMRS enables efficient metabolic flux analysis without requiring specialized equipment.