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Updated: May 5, 2026

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Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
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Differential melting voltage by tandem-trapped ion mobility spectrometry: glycan structure influences glycoprotein
Mengqi Chai1, Christian Bleiholder1,2, Fanny C Liu1
1Department of Chemistry and Biochemistry, Florida State University, 102 Varsity Way Tallahassee Florida 32306 USA fliu@fsu.edu.
RSC Chemical Biology
|September 2, 2025
Abstract:
Profiling the full spectrum of protein glycoforms is critical to understanding their functional roles. We developed the differential melting voltage approach using tandem-ion mobility/tandem-mass spectrometry and applied it to study Ribonuclease B glycoforms. Our results indicate that, in addition to glycan mass and intact protein size, the glycan structure plays a role in regulating the stability of Ribonuclease B.

