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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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On-Target Photoassembly of Pyronin Dyes for Super-Resolution Microscopy.

Gergely Knorr1, Mariano L Bossi1, Stefan W Hell1,2

  • 1Department of Optical Nanoscopy, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany.

Angewandte Chemie (International Ed. in English)
|September 10, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed photoactivatable fluorescent markers called Pyronin Upon Light Irradiation (PULI) by modifying pyronin molecules. These PULI markers overcome nuclear accumulation issues, enabling advanced super-resolution microscopy imaging.

Keywords:
DyesFluorescencePhotoactivationPyroninSuper‐resolution microscopy

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Area of Science:

  • Chemical Biology
  • Microscopy
  • Biophysics

Background:

  • Pyronins are useful fluorophores but their nuclear accumulation limits super-resolution microscopy.
  • Controlled photoactivation offers low background signal for microscopy.

Purpose of the Study:

  • To design novel photoactivatable fluorescent markers based on pyronins.
  • To overcome the limitations of traditional pyronins for super-resolution imaging.

Main Methods:

  • Synthesis of diaryl ether and diaryl silane molecules.
  • Photoactivation of synthesized molecules into fluorescent (silicon-)pyronins (PULI).
  • Application of PULI markers in super-resolution microscopy techniques (PALM, STED, MINFLUX).

Main Results:

  • Developed PULI molecules that become fluorescent upon photoactivation.
  • Demonstrated PULI markers overcome nuclear accumulation issues.
  • Extended the PULI concept to far-red emitting silicon-bridged analogues.
  • Successfully imaged diverse biological targets in cells using advanced microscopy.

Conclusions:

  • PULI represents a versatile platform for photoactivatable fluorescent markers.
  • This approach enables super-resolution imaging with improved specificity and reduced background.
  • The developed molecules offer a promising alternative to existing fluorescent probes.