High-performance liquid chromatography of linoleic acid hydroperoxides and their corresponding alcohol derivatives

The four major hydroperoxides derived from autoxidation or lipoxygenase action on linoleic acid or on methyl linoleate and their corresponding alcohol derivatives are resolved by high-performance liquid chromatography on Zorbax SIL 3 micron particulate columns irrigated with hexane-diethyl ether-acetic acid mixtures. The four major linoleic acid hydroperoxides are interconverted at different rates in benzene or carbon tetrachloride solutions but are stable to storage under nitrogen at -70 degrees C for several months.