Related Experiment Video
Updated: May 1, 2026
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Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
Published on: February 25, 2007
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Protocol for chromatin immunoprecipitation and hematovascular function assays with differentiated mouse embryonic
Raham Lee1, Minseong Kim1, Changwon Park1
1Department of Molecular and Cellular Physiology, Louisiana State University Health Science Center, Shreveport, LA 71103, USA.
STAR Protocols
|September 12, 2025
Summary
This protocol outlines mouse embryonic stem cell (mESC) differentiation into embryoid bodies (EBs) to study hematovascular development. It details methods for assessing epigenetic modifications and lineage commitment during stem cell differentiation.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Epigenetics
Background:
- Mouse embryonic stem cells (mESCs) are a key model for studying early development.
- Understanding stem cell differentiation into specific lineages is crucial for regenerative medicine.
Purpose of the Study:
- To present a detailed protocol for differentiating mESCs into embryoid bodies (EBs).
- To enable the investigation of epigenetic modifications during hematovascular lineage commitment.
Main Methods:
- Step-by-step differentiation of mESCs into EBs.
- Single-cell dissociation and chromatin preparation for ChIP (crosslinking, sonication).
- Assays for hematovascular differentiation: EB vascular sprouting, hematopoietic colony formation, and flow cytometry.
Main Results:
- The protocol facilitates the study of dynamic interplay between epigenetic modifications and lineage commitment.
- It provides a comprehensive framework for analyzing hematovascular differentiation from mESCs.
Conclusions:
- This protocol serves as a valuable tool for researchers studying stem cell differentiation and developmental processes.
- It enables in-depth analysis of epigenetic regulation in hematovascular development.
