Related Experiment Video
Updated: Jan 18, 2026

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
Published on: July 16, 2013
Study on the Calcium Transport-Promoting Property and Mechanism of the Peptide-Calcium Complex DEEENDQVK-Ca Based on
Yaxin Zhang1, Jingjing Ru1, Shan Gao1
1Tianjin Key Laboratory of Food Quality and Health, College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China.
Abstract:
Peptide-calcium complexes exhibit promise as calcium supplements due to their enhanced bioavailability. Phosvitin nonapeptide DEEENDQVK (DK) possesses a high calcium-binding capability. This study investigated the calcium transport-promoting properties of DK and DEEENDQVK-calcium complex (DK-Ca) using a Caco-2 monolayer model. Both DK and DK-Ca concentration-dependently promoted calcium transport, and compared with the CaCl2 control, enhanced calcium transport by 1.07-fold and 1.83-fold, respectively. The calcium transport channels and mechanisms of DK and DK-Ca were also investigated using transfer channel regulators, real-time quantitative polymerase chain reaction, and molecular docking. The results showed that DK-Ca primarily promoted calcium transport through the TRPV6 channel, with supplementary contributions from paracellular and endocytosis channels, while DK did not rely on the endocytosis channel. DK and DK-Ca promoted calcium transport by upregulating TRPV6, calbindin-D9k, PMCA1b, and claudin-2 mRNA expression. DK-Ca exhibited a higher binding affinity for TRPV6 (-10.0 kcal/mol) compared to DK (-5.5 kcal/mol). DK-Ca primarily binds TRPV6's extracellular exposed cavity through hydrogen bonds and hydrophobic interactions, increasing the local calcium concentration at the channel entrance to promote calcium absorption. This study provides cellular-level mechanistic clues for the potential effects of DK-Ca as a calcium supplement.
Related Concept Videos
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Synthesis and Functions of Calcitonin
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...
Drug Absorption Mechanism: Carrier-Mediated Membrane Transport
Facilitated diffusion is a passive process that utilizes human Solute Carrier (SLC) transporters. These transporters bind to the drug, undergo structural...
Overview of Secretory Vesicles
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Drug Absorption Mechanism: Passive Membrane Transport

