Related Experiment Video
Updated: Jan 17, 2026
04:55
A Stably Established Two-Point Injection of Lysophosphatidylcholine-Induced Focal Demyelination Model in Mice
Published on: May 11, 2022
4.7K
NLRP2 Immunoreactivity in Mouse Brain After Cortical Spreading Depolarization
Eda Derle1,2, Buket Dönmez Demir2, Melike Sever Bahçekapılı2
1Department of Neurology, Başkent University Faculty of Medicine, Ankara, Türkiye.
Noro Psikiyatri Arsivi
|September 15, 2025
Summary
Cortical spreading depolarization (CSD) significantly reduces NLRP2 expression in brain tissue. This study investigated NLRP2 alterations in neurons and astrocytes following CSD.
Area of Science:
- Neuroscience
- Molecular Biology
Background:
- NLRP2 is a component of the inflammasome.
- Its role in cortical spreading depolarization (CSD) is not well understood.
Purpose of the Study:
- To investigate the expression pattern of NLRP2.
- To determine alterations in NLRP2 following a single CSD event.
Main Methods:
- Brain tissue was collected 15 minutes after CSD induction in mice.
- Immunofluorescence staining was used to examine NLRP2 expression.
- Dual staining identified NLRP2-expressing cells (neurons and astrocytes).
Main Results:
- NLRP2 immunoreactivity was observed in cortical and subcortical regions of control and naive brains.
- A notable reduction in NLRP2 immunoreactivity was found in CSD-induced brains.
- Most NLRP2-expressing cells were neurons (NeuN+), with a smaller population in astrocytes (S100β+).
Conclusions:
- Single CSD leads to decreased NLRP2 immunoreactivity in the early stages.
- NLRP2 is expressed in both neurons and astrocytes in the brain.
