DNA-Guided Multivalent Antibody Display Boosts the Targeting Performance of Therapeutic Nanovesicles

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Immunology

Background:

• Nanovesicles (NVs) are emerging as promising alternatives to extracellular vesicles in biomedical applications.
• Clinical translation of NVs is limited by insufficient targeting specificity for diseased tissues.

Purpose of the Study:

• To develop a DNA-mediated self-assembly strategy for creating multivalent antibody-modified nanovesicles (Multi-NVs).
• To enhance targeted delivery of NVs to specific diseased tissues.

Main Methods:

• Utilized a DNA-mediated self-assembly approach to construct Multi-NVs.
• Modified nanovesicles with multivalent antibodies for improved targeting.
• Evaluated Multi-NVs derived from mesenchymal stromal cells in disease models.

Main Results:

• Multi-NVs demonstrated robust and specific targeting capabilities.
• Achieved improved cellular uptake efficiency and promoted endothelial angiogenic activity.
• Showed broad applicability in acute lung injury (ALI) and acute kidney injury models with enhanced tissue targeting.
• Therapeutic treatment with Multi-NVs reduced macrophage infiltration and alleviated lung tissue damage in ALI.

Conclusions:

• The modular DNA-mediated strategy enables the construction of highly specific nanovesicle-based drug delivery systems.
• Multi-NVs show significant potential for precision medicine and personalized therapeutic interventions in various disease contexts.