Study of the L-Carnitine Effect on Myocardial Bioelectrical Activity by Microelectrode Arrays and Artificial...

Area of Science:

• Cardiovascular Physiology
• Artificial Intelligence in Medicine
• Biomedical Signal Processing

Background:

• L-carnitine is crucial for fatty acid oxidation but its cardiovascular effects remain unclear.
• Understanding L-carnitine's impact on heart function is vital for cardiovascular health.
• Microelectrode array technology provides detailed myocardial bioelectrical activity data.

Purpose of the Study:

• To investigate the effects of L-carnitine on the cardiovascular system using AI analysis.
• To develop and apply a novel neural network for electrogram analysis.
• To assess the potential clinical applicability of the developed methodology.

Main Methods:

• Analysis of multichannel electrograms from isolated rat hearts using AI algorithms.
• Development of a U-Net based neural network for 1D signal segmentation of activation times.
• Ex vivo experimentation on rat myocardium with L-carnitine and adrenaline administration.

Main Results:

• L-carnitine administration resulted in decreased heart rate and myocardial excitation-conduction velocity.
• Reduced intensity of coronary blood flow was observed following L-carnitine treatment.
• The physiological cardiac response to adrenaline was diminished by L-carnitine.

Conclusions:

• L-carnitine exhibits inhibitory effects on cardiac function and response to adrenergic stimulation.
• The AI-based electrogram analysis methodology shows promise for clinical translation in human electrophysiology.
• Further research is warranted to elucidate the precise mechanisms of L-carnitine's cardiovascular actions.