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Immunogold Electron Microscopy

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Updated: May 2, 2026

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

Published on: June 11, 2013

Light-Responsive Liquid Metal Particles for Nucleic Acid Extraction and Directional Enrichment.

Shuyue Jiang^1,2,3, Donghao Cui^1,3, Enjia Zhang¹

¹State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.

Small (Weinheim an Der Bergstrasse, Germany)

|September 19, 2025

Summary

This summary is machine-generated.

New TiO2@Ga particles offer rapid viral lysis and nucleic acid extraction for faster COVID-19 diagnostics. This advancement simplifies sample preparation, improving point-of-care molecular testing capabilities.

Keywords:

liquid metal nucleic acid extraction photoactive particles self‐propulsion virus lysis

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Last Updated: May 2, 2026

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

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Published on: June 11, 2013

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Published on: January 20, 2016

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

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Published on: April 15, 2021

Area of Science:

Biomaterials Engineering
Nanotechnology
Molecular Diagnostics

Background:

Conventional quantitative polymerase chain reaction (qPCR) diagnostics face challenges including time-consuming nucleic acid extraction and reliance on skilled operators.
The COVID-19 pandemic highlighted the need for rapid, efficient, and user-friendly diagnostic methods.

Purpose of the Study:

To develop novel photoactive titanium dioxide@gallium (TiO2@Ga) particles for integrated viral lysis, nucleic acid extraction, and enrichment.
To evaluate the efficiency of TiO2@Ga particles in enhancing viral lysis and nucleic acid capture for molecular diagnostics.

Main Methods:

Fabrication of photoactive TiO2@Ga particles.
Utilizing ultrasound and liquid metal gallium (Ga) for enhanced viral lysis and reactive oxygen species (ROS) generation.
Employing UV irradiation for photocatalytic propulsion and nucleic acid capture by TiO2.
Assessing particle migration velocity under UV light.
Evaluating qPCR performance (Ct values) with TiO2@Ga particles for SARS-CoV-2 plasmids and pseudoviruses.
Demonstrating nucleic acid extraction and transport in a microfluidic chip.

Main Results:

TiO2@Ga particles demonstrated enhanced viral lysis and nucleic acid capture, evidenced by lower qPCR cycle threshold (Ct) values compared to controls.
Particles achieved migration velocities up to 6 µm/s under specific UV conditions.
Efficient extraction and transport of viral nucleic acids were confirmed within a single-channel microfluidic chip.
The integrated system showed potential for rapid molecular diagnostics.

Conclusions:

The developed TiO2@Ga particles provide a rapid, integrated solution for viral lysis and nucleic acid extraction.
This technology significantly improves efficiency and user-friendliness for molecular diagnostics.
The approach shows strong potential for advancing point-of-care diagnostic applications.