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Updated: Jan 17, 2026

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
Published on: November 23, 2021
Recent advances in fluorescent glycoconjugate probes for biosensing, bioimaging and targeted photodynamic therapy
Hai-Hao Han1,2,3, Xi-Le Hu1, Xiao-Peng He1,4
1Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, China. xphe@ecust.edu.cn.
Abstract:
Fluorescent glycoconjugate probes have emerged as innovative tools for biomedical research, helping to delineate the mechanistic roles of sugars in biological and pathological events. This feature article reviews the recent advances in the development and application of fluorescent glycoconjugate probes since 2021, highlighting their applications for biosensing, targeted imaging and phototherapy. We describe the design principles of these probes, their utilization in the detection and cell imaging of glycosidase activities, and their potential to be developed as theranostic agents. We also discuss the challenges and future directions in this field, including the need for the development of sensitive, selective and biocompatible probes for early diagnosis and treatment of major human diseases such as cancer.
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