Related Experiment Video
Updated: May 6, 2026
Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model
Published on: August 4, 2017
Engineered Spider Silk in Core-Shell Multifunctional Fibrous Mat for Accelerated Chronic Diabetic Wound Healing via
Mercyjayapriya Jebakumar1,2, Mohandass Pachaiyappan1,2, Numbi Ramudu Kamini1,2
1Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research (CSIR)─Central Leather Research Institute (CLRI), Chennai, Tamil Nadu 600020, India.
A new silk DOPA-crisaborole conjugate and eugenol biomaterial (Dex-SDC/PCL-Eu) effectively treats chronic diabetic wounds by modulating macrophages and reducing oxidative stress. This promotes faster healing with improved tissue regeneration and collagen deposition.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing Research
Background:
- Chronic diabetic wounds exhibit impaired macrophage polarization (M1 to M2), leading to increased reactive oxygen species (ROS), reduced angiogenesis, and poor collagen synthesis.
- Current treatments often fail to address the complex inflammatory and oxidative environment of these wounds.
Purpose of the Study:
- To develop a multifunctional biomaterial capable of modulating the immune microenvironment and combating ROS in chronic diabetic wounds.
- To investigate the therapeutic potential of a novel combination therapy using silk DOPA-crisaborole conjugate (SDC) and eugenol (Eu) for enhanced wound healing.
Main Methods:
- Fabrication of a core-shell electrospun fibrous mat (Dex-SDC/PCL-Eu) with controlled release of crisaborole and eugenol.
- In vitro assessment of macrophage polarization (IL-10, IL-6 expression) and cellular responses (fibroblast/endothelial cell behavior).
- In vivo evaluation in a diabetic rat model to assess wound closure, re-epithelialization, neovascularization, and collagen deposition.
Main Results:
- Dex-SDC/PCL-Eu successfully induced M1 to M2 macrophage polarization, upregulating IL-10 and downregulating IL-6.
- Sustained release of eugenol effectively mitigated oxidative damage.
- In vitro studies showed enhanced fibroblast and endothelial cell proliferation and migration, with improved tube formation.
- In vivo studies demonstrated accelerated wound healing, reduced inflammation, and promoted organized tissue regeneration with uniform collagen deposition in diabetic rats.
Conclusions:
- The multifunctional Dex-SDC/PCL-Eu fibrous mat effectively promotes chronic diabetic wound healing.
- This novel biomaterial demonstrates potential for treating complex, non-healing wounds by addressing key pathological mechanisms like inflammation and oxidative stress.
