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Updated: Jan 17, 2026

An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse
Published on: September 12, 2018
Pinpointing a leaky bleb with a blue surgical skin marker
Sarang P Lambat1, Nivea Surana, Prabhat V Nangia
1Department of Glaucoma, Suraj Eye Institute, Nagpur, Maharashtra, India.
None:
We document a new technique to identify the site of leak from the entry point following bleb needling using a surgical skin marker. A 67-year-old man with advanced glaucoma in the right eye underwent trabeculectomy followed by bleb needling for subconjunctival fibrosis. Subsequently, he developed a bleb leak from the needling entry point with an intraocular pressure of zero mmHg. Despite pressure bandage application for 24 hours, the posteriorly located leak persisted. Suturing of the leakage point was recommended due to persistent hypotony. During surgery, the leakage points were not visualized. We stained the conjunctiva with a surgical skin marker at the suspected leakage site, which highlighted two closely situated openings and facilitated accurate suture placement. Complete closure of the leaks was achieved, resulting in an elevated bleb postoperatively. This is a simple and inexpensive method to identify the site of a leaking bleb under a microscope.

