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Related Experiment Videos

Antibodies specific to a deoxyribodinucleotide sequence.

S A Khan, M Z Humayun, T M Jacob

    Nucleic Acids Research
    |September 1, 1977
    PubMed
    Summary
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    Researchers developed antibodies specific to the dinucleotide sequence deoxyadenylyl-deoxyadenylyl-thymidine (dpApT). These antibodies specifically bind to dpApT in synthetic DNA and denatured calf thymus DNA.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Biochemistry

    Background:

    • Antibodies are crucial tools for detecting specific molecular targets.
    • Hapten-specific antibodies are valuable for studying nucleic acid structures.

    Purpose of the Study:

    • To generate and characterize antibodies specific to the dinucleotide sequence deoxyadenylyl-deoxyadenylyl-thymidine (dpApT).
    • To assess the specificity and binding capabilities of the generated antibodies.

    Main Methods:

    • Antibody generation in rabbits against a dpApT-bovine serum albumin conjugate.
    • Double diffusion in agar gel and quantitative precipitation tests.
    • Nitrocellulose membrane binding assay to quantify antibody-antigen interactions and specificity.

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    Main Results:

    • Antibodies specific to the dpApT hapten were successfully generated.
    • The antibodies demonstrated high specificity for the dinucleotide sequence dpApT.
    • Binding inhibition assays confirmed specificity against various oligonucleotides.
    • Antibodies bound to synthetic oligonucleotides containing dpApT and denatured calf thymus DNA.

    Conclusions:

    • Rabbit antisera contain antibodies highly specific for the dpApT dinucleotide sequence.
    • These antibodies can recognize and bind to specific DNA sequences within larger DNA molecules.