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Amplification of Ultra-Trace DNA from Early Sheep Embryos Based on qPCR: Establishing a Gender Identification System
Peng Niu1, Weikun Tao2, Fei Huang1
1College of Life Science and Technology, Tarim University, Alar 843300, China.
Biology
|September 27, 2025
Summary
This study developed a rapid and accurate method for sexing sheep embryos using qPCR targeting the SRY gene. This technique offers a reliable solution for livestock breeding by identifying embryo sex with 100% accuracy.
Area of Science:
- Animal Science
- Molecular Biology
- Genetics
Background:
- Sex identification in mammalian embryos is crucial for livestock production.
- Current methods for embryo sex determination are often costly and unreliable.
- A simple, fast, and dependable method is needed for commercial applications.
Purpose of the Study:
- To develop and optimize a novel method for sexing sheep embryos.
- To establish a reliable and cost-effective technique for identifying embryo sex in vitro.
- To improve livestock breeding efficiency through accurate sex determination.
Main Methods:
- Designed primer probes targeting the SRY gene on the Y chromosome.
- Utilized quantitative Polymerase Chain Reaction (qPCR) to amplify SRY and GAPDH genes.
- Optimized the detection system using ultra-trace amounts of genomic DNA from sheep blood and embryos.
Main Results:
- Male embryos showed amplification bands for both SRY and GAPDH genes.
- Female embryos exhibited amplification bands only for the GAPDH gene.
- The established system achieved 100% accuracy in sheep embryo sex identification.
Conclusions:
- A highly sensitive and accurate qPCR-based method for sheep embryo sex determination was successfully established.
- The system demonstrated a sensitivity as low as 5.64 pg of DNA.
- This technique provides a valuable tool for commercial livestock production and genetic improvement.
