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Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
Published on: June 25, 2018
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Structural Elucidation and Storage Stability of Novel Dietary Sulfur Compounds from Radish
Gyeong A Jeong1, Inae Lee1,2, Jeehye Sung3
1Department of Food Science and Biotechnology, Wonkwang University, Iksan 54538, Jeonbuk, Republic of Korea.
Foods (Basel, Switzerland)
|September 27, 2025
Summary
Researchers extracted and purified sulforaphane-derived compound X (SFN-DX) from radishes. Optimal storage at -18°C preserves SFN-DX stability, crucial for its functional food applications.
Area of Science:
- Nutritional biochemistry and functional foods
- Phytochemistry and natural product isolation
Background:
- Radishes (Raphanus sativus L.) are recognized for bioactive compounds, including sulforaphane (SFN), known for antioxidant and anticancer properties.
- Dietary sulfur-containing bioactive compounds, like SFN derivatives, are of interest for their health benefits.
Purpose of the Study:
- To extract, purify, and identify SFN and a novel SFN-derived compound X (SFN-DX) from freeze-dried radishes.
- To evaluate the storage stability of purified SFN-DX under various conditions.
Main Methods:
- High-performance liquid chromatography (HPLC) for quantification and initial analysis of SFN-DX in radish extract.
- Preparative liquid chromatography for isolating SFN-DX to high purity (99%).
- Structural elucidation techniques to confirm the identity of SFN-DX as an SFN derivative.
- Stability testing of SFN-DX at temperatures ranging from -18°C to 40°C over different storage durations.
Main Results:
- Freeze-dried radish extract contained 13.262 mg/g of SFN-DX, with HPLC analysis showing a retention time close to the SFN standard.
- SFN-DX was successfully isolated with 99% purity, and structural elucidation confirmed it as an isothiocyanate-like SFN derivative.
- SFN-DX purity significantly decreased at elevated temperatures (20°C and 40°C) and with prolonged storage; -18°C was identified as the optimal storage temperature.
Conclusions:
- This study provides a scientific foundation for the efficient extraction and characterization of SFN-DX from radishes.
- Optimal storage conditions (-18°C) are essential for maintaining the stability and integrity of SFN-DX for its use as a functional food ingredient.
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