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In Vitro Assessment of Electrospun PVP+AgNPs Scaffolds for Bioactive Medical Use.
Ileana Ielo1, Luana Vittoria Bauso1, Antonio Laezza2
1Department of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
International Journal of Molecular Sciences
|September 27, 2025
Summary
This study developed novel polyvinylpyrrolidone (PVP) scaffolds with silver nanoparticles (AgNPs) for advanced wound healing. These bioactive platforms show promise for tissue regeneration and combating microbial infections in chronic wounds.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Regenerative Medicine
Background:
- Chronic wounds and post-operative complications present significant biomedical challenges, including impaired tissue regeneration and persistent, antibiotic-resistant microbial infections.
- Biofilm formation exacerbates infection, hindering effective treatment and increasing the need for advanced therapeutic strategies.
- Current treatments often struggle to address both tissue repair and infection control simultaneously.
Purpose of the Study:
- To develop and evaluate electrospun polyvinylpyrrolidone (PVP) scaffolds embedded with silver nanoparticles (AgNPs) as multifunctional platforms.
- To assess the potential of these PVP+AgNPs scaffolds for wound healing and implant applications.
- To investigate the synergistic effects of AgNPs and PVP on tissue regeneration and antimicrobial activity.
Main Methods:
- Synthesis of silver nanoparticles (AgNPs) and their incorporation into a polyvinylpyrrolidone (PVP) matrix via optimized electrospinning.
- Structural and mechanical characterization using Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).
- In vitro evaluation including cytotoxicity assays with fibroblast cell lines and antibiofilm activity against *Staphylococcus aureus*.
Main Results:
- Characterization confirmed homogenous dispersion of AgNPs within the PVP matrix and favorable mechanical properties.
- In vitro cytotoxicity assays demonstrated good biocompatibility with fibroblast cell lines.
- Significant inhibition of *Staphylococcus aureus* biofilm formation was observed, indicating potent antimicrobial efficacy.
Conclusions:
- Electrospun PVP+AgNPs scaffolds show strong potential as multifunctional biomaterials for wound healing and implant coatings.
- The scaffolds exhibit a synergistic capacity to support tissue regeneration and inhibit microbial infections.
- Further in vitro and in vivo studies are warranted to confirm therapeutic efficacy, biocompatibility, and long-term stability.
