Protocol for trapping transient endogenous formaldehyde in live cells to visualize its signaling
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Updated: Aug 7, 2026

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
Published on: November 7, 2013
Yuan Pan1, Xing-Guang Liang2, Xin Li3
1College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Abstract:
Formaldehyde is emerging as a key signaling modulator. Here, we present Trami, a protocol that traps endogenous formaldehyde in live cells and converts its transient signals into stable proximal protein labels for subsequent signaling study. We describe probe design, live-cell administration, and imaging procedures for signaling deconvolution. Trami offers a powerful tool for elucidating formaldehyde-mediated signaling pathways. For complete details on the use and execution of this protocol, please refer to Pan et al.1.
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