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Published on: March 21, 2018
Aggregation-induced electrochemiluminescence aptasensor for ultrasensitive detection of acetamiprid based on polymer
Yuanyuan Jin1, Yerong Zhao1, Haibing Zhu1
1School of Chemistry and Materials, Yangzhou University, Yangzhou, 225002, China.
Abstract:
Acetamiprid (ACE) poses significant threats to the environment and human health. Thus, the development of sensitive ACE detection is crucial for safeguarding human health. Herein, we developed an aggregation-induced electrochemiluminescence (AIECL) aptasensor for ACE detection, in which polymer dots (Pdots) are used as luminescent probe. By virtue of the electrochemiluminescence-resonance energy transfer (ECL-RET) mechanism, ECL signal of Pdots is quenched by black hole quencher (BHQ) linked to ACE aptamer, thereby turning "off" the signal of sensor. Upon the existence of ACE, BHQ group releases from the Pdots and escapes from the surface of sensor, consequently enabling the restoration of ECL signal of Pdots. Such sensor exhibits excellent analysis performance with a lower detection limit of 9.1 aM, over conventional ECL analysis for ACE. Significantly, the sensor is applied to lettuce sample with good recovery rate. This work provides an effective method for ACE detection, and validates its application potential in food safety supervision.
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