Characterization of Transgenic Plants by Southern Blotting in Rice

This study investigates the genomic organization of the OsSAMDC gene in rice, focusing on its presence in the transgenic japonica rice cultivar Yukihikari. Using Southern blotting, the research examines the number and arrangement of OsSAMDC-like genes in the transgenic rice genome. The methodology involves extracting high-molecular-weight genomic DNA, digesting it with various restriction enzymes and performing Southern hybridization analysis. Multiple hybridization probes, including a full-length cDNA and gene-specific fragments, are employed to study the genomic organization of OsSAMDC and related genes. DNA sequencing and bioinformatics analyses provide insights into the gene's structure and potential gene family members. This comprehensive approach combines molecular biology techniques to elucidate the genomic context of OsSAMDC in transgenic rice, potentially contributing to our understanding of polyamine biosynthesis genes and their role in stress tolerance.