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Updated: Jan 16, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
Transformation of Pichia pastoris by Electroporation
Kirill Smirnov1, Anton Glieder2
1Christian Doppler Laboratory for Innovative Pichia pastoris Host and Vector Systems, Graz University of Technology, Petersgasse, Graz, Austria. k.smirnov@tugraz.at.
Abstract:
Transformation is one of the cornerstone techniques of molecular biotechnology, and it is an almost obligatory part of any study related to the investigation of gene functions, the production of recombinant proteins, or the alterations of the phenotypic features in Komagataella phaffii. One of the most commonly used transformation methods for delivering foreign DNA to K. phaffii cells is electroporation.This chapter provides a simplified and fast protocol for preparing K. phaffii electrocompetent cells and for their transformation by electroporation. In addition, several parameters affecting the transformation efficiency are briefly described.
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