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Updated: May 6, 2026
Preparation of Acute Subventricular Zone Slices for Calcium Imaging
Published on: September 19, 2012
Calcium Imaging Studies in Enteric Glia
Beatriz Thomasi1, Julia Jamka2, Brian D Gulbransen2
1Department of Physiology, Michigan State University, East Lansing, MI, USA. thomasi7@msu.edu.
Abstract:
Calcium (Ca2+) imaging is a versatile technique to study cellular activity and is a particularly important tool to study electrically passive cells such as glia. Enteric glia play important roles in gut physiology, and their activity can be captured using Ca2+ imaging. Ca2+-encoded activities command glial processes such as gliotransmission and intercellular signaling. This chapter describes a protocol that will allow users to study glial Ca2+ activity in ex vivo whole-mounts of circular muscle-myenteric plexus. This chapter will include comprehensive steps to execute Ca2+ imaging experiments, data extraction, and analysis.
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