Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

32.1K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.1K
Embryonic Stem Cells00:57

Embryonic Stem Cells

4.7K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
4.7K
Adult Stem Cells01:33

Adult Stem Cells

33.4K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.4K
Stem Cell Culture01:17

Stem Cell Culture

6.0K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
6.0K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

27.2K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
27.2K
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

6.5K
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
6.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neural and Genetic Mechanisms Regulating Copulation Latency in Male Drosophila melanogaster.

Genetics·2026
Same author

Reconstituting human primitive streak formation through extra-embryonic cell coordination.

Cell·2026
Same author

Depicting the dynamic transcriptional and epigenetic landscape of testis development in pubertal Simmental cattle.

Journal of animal science and biotechnology·2026
Same author

Cannabidiol triggers fatty acids β-oxidation mediated by Stat2 to facilitate intestinal stem cells regeneration post radiation.

Experimental & molecular medicine·2026
Same author

A three-dimensional spatial transcriptome atlas reconstructs early organogenesis in primate Carnegie stages 9 and 10 embryos.

Nature cell biology·2026
Same author

Human stem cell-based embryo models: innovation, ethics, and policy.

Human reproduction (Oxford, England)·2026
Same journal

Patient-derived organoids reveal ductal dysfunction and CFTR-modulator responses in chronic pancreatitis.

Cell stem cell·2026
Same journal

Lineage plasticity driven by GATA6 loss fuels colorectal cancer metastasis.

Cell stem cell·2026
Same journal

Quantitative molecular cartography of emergency myelopoiesis reveals conserved modules of hematopoietic activation.

Cell stem cell·2026
Same journal

Paired pre- and post-transplant human immunoprofiling identifies an IFN-γ-JAK1 axis limiting stem-cell-derived RPE engraftment.

Cell stem cell·2026
Same journal

ENPP1 blockade with a humanized monoclonal antibody enhances renal repair after acute kidney injury.

Cell stem cell·2026
Same journal

ZNF512B safeguards genome integrity at regulatory regions to repress the SASP and inflammation.

Cell stem cell·2026
See all related articles

Related Experiment Video

Updated: Jan 16, 2026

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

7.3K

Building the start: Unlocking advanced stem cell-based embryo models.

Zekun Wu1, Hongan Ren1, Leqian Yu1

  • 1State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Cell Stem Cell
|October 3, 2025
PubMed
Summary
This summary is machine-generated.

Researchers optimized chemical induction to derive extraembryonic lineages from embryonic stem cells (ESCs) without transgenes. This breakthrough enables reproducible mouse stem cell-based embryo models (SCBEMs) to develop to later stages.

More Related Videos

Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions
05:13

Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions

Published on: June 21, 2024

1.6K
Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
12:59

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

9.0K

Related Experiment Videos

Last Updated: Jan 16, 2026

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

7.3K
Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions
05:13

Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions

Published on: June 21, 2024

1.6K
Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
12:59

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

9.0K

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Genetics

Background:

  • Embryonic stem cells (ESCs) are crucial for developmental studies.
  • Deriving extraembryonic lineages from ESCs traditionally requires transgenes.
  • Previous models had limitations in developmental progression.

Purpose of the Study:

  • To develop a transgene-free method for deriving extraembryonic lineages from ESCs.
  • To establish reproducible mouse stem cell-based embryo models (SCBEMs).
  • To advance SCBEMs to later developmental stages.

Main Methods:

  • Optimized chemical induction protocols.
  • Refined culture conditions for ESCs.
  • Utilized transgene-free approaches.

Main Results:

  • Successfully derived extraembryonic lineages directly from ESCs.
  • Achieved reproducible development of SCBEMs.
  • Enabled SCBEMs to advance to the E8.5-E8.75 stage.

Conclusions:

  • Transgene-free derivation of extraembryonic lineages is feasible.
  • Optimized chemical induction and culture conditions are key.
  • This method advances the development of SCBEMs for research.