Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Structure of Lipids03:38

Structure of Lipids

98.1K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
98.1K
Structure of Lipids03:38

Structure of Lipids

13.8K
13.8K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

4.7K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
4.7K
Phase Transitions: Melting and Freezing02:39

Phase Transitions: Melting and Freezing

14.6K
Heating a crystalline solid increases the average energy of its atoms, molecules, or ions, and the solid gets hotter. At some point, the added energy becomes large enough to partially overcome the forces holding the molecules or ions of the solid in their fixed positions, and the solid begins the process of transitioning to the liquid state or melting. At this point, the temperature of the solid stops rising, despite the continual input of heat, and it remains constant until all of the solid is...
14.6K
Fats as Energy Storage Molecules01:06

Fats as Energy Storage Molecules

26.8K
Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis...
26.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interfacial properties of plant protein-flavonoid hybrid particles: towards influencing in vitro gastric digestion of emulsions.

Food chemistry·2026
Same author

Decellularized plant scaffolds in food engineering: a comprehensive review of techniques, characterization, and potential in alternative meat applications.

Food chemistry·2026
Same author

A novel, single-step LC-ELSD method to characterize the aliphatic fraction of natural waxes.

Food chemistry·2026
Same author

Investigating the Interfacial Structure of Potato Protein Microgels at the Air-Water Interface.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Versatile high-temperature heating system for drying droplets in the TinyLev acoustic levitator.

The Review of scientific instruments·2025
Same author

Internally Self-Assembled Pickering Mesosomes Stabilized by Positively Charged Lactoferrin.

Langmuir : the ACS journal of surfaces and colloids·2025

Related Experiment Video

Updated: Jan 16, 2026

A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients
11:27

A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients

Published on: August 9, 2022

2.4K

Polymorphic Structures and Transitions of Triglycerides and Complex Fats: A Guide to X-Ray Scattering Approaches.

Julia Seilert1, Megan Holdstock2, Yoga Pratama2,3

  • 1Department of Food Process Engineering, Technische Universität Berlin, Berlin, Germany.

Comprehensive Reviews in Food Science and Food Safety
|October 5, 2025
PubMed
Summary

This review details X-ray scattering methods for analyzing triglyceride nanostructure and crystallization. It offers practical tools to evaluate polymorphic states and phase transitions in fats for industrial and academic research.

Keywords:
X‐ray scatteringphase transitionpolymorphssolid fat contenttriglycerides

More Related Videos

Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
07:12

Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling

Published on: August 23, 2024

1.8K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.3K

Related Experiment Videos

Last Updated: Jan 16, 2026

A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients
11:27

A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients

Published on: August 9, 2022

2.4K
Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
07:12

Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling

Published on: August 23, 2024

1.8K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.3K

Area of Science:

  • Materials Science
  • Physical Chemistry
  • Crystallography

Background:

  • Triglycerides form complex nanostructures crucial for fat properties.
  • Understanding triglyceride crystallization is key to controlling fat behavior.
  • Existing methods for analyzing fat nanostructure are often limited.

Purpose of the Study:

  • To provide a comprehensive review of nanostructural aspects in pure triglycerides and complex fat systems.
  • To offer a practical guide for utilizing X-ray scattering techniques.
  • To present robust methods for evaluating polymorphic states and phase transitions in fats.

Main Methods:

  • Review of public-domain X-ray scattering data for various triglycerides.
  • Analysis of nanoscale features like lamellar stacking and hydrocarbon tilt angles.
  • Application of tools for electron density profile calculations, chain tilt angle estimation, crystallite size/strain analysis, and area per hydrocarbon chain determination.

Main Results:

  • Detailed explanation of nanoscale lamellar stacking and hydrocarbon tilt angles.
  • Insights into molecular-scale chain packing density and geometry changes.
  • Summarized crystallographic data including long- and short-spacing.
  • Demonstrated generalization of model-free methods to analyze complex fat systems and solid fat content.

Conclusions:

  • X-ray scattering provides powerful tools for detailed evaluation of triglyceride nanostructure.
  • The reviewed methods enable robust analysis of polymorphic states and phase transitions.
  • This work supports research at the interface of academia and industry concerning fat science.