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Updated: Jan 15, 2026
09:40
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
Published on: February 6, 2018
15.7K
DNAH10 interacts with UCHL3-PACRG complex to coordinate sperm head and flagella development during spermiogenesis
Rui Zheng1, Jierui Yan1, Yuting Wen1
1Joint Lab of Reproductive Medicine of SCU-CUHK, Lab of Reproductive Genetics and Epigenetics, Department of Obstetrics/Gynecology, Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
Development (Cambridge, England)
|October 8, 2025
Summary
Dynein axonemal heavy chain 10 (DNAH10) deficiency causes male infertility by impairing sperm morphology and function. DNAH10 is crucial for intra-manchette transport, impacting intra-manchette transport and offering insights for infertility diagnosis.
Area of Science:
- Male reproductive biology
- Spermatozoa
- Cellular transport mechanisms
Background:
- Asthenoteratozoospermia, a cause of male infertility, is linked to dynein protein dysfunction.
- Dynein axonemal heavy chain 10 (DNAH10) has been previously associated with asthenoteratozoospermia, but its mechanism remains unknown.
Purpose of the Study:
- To elucidate the functional role of DNAH10 in sperm development and male fertility.
- To investigate the molecular mechanism by which DNAH10 influences sperm morphology and function.
Main Methods:
- Investigated the effects of DNAH10 deficiency on sperm morphology and flagella.
- Analyzed the impact of DNAH10 dysfunction on manchette function and axonemal protein localization.
- Examined the interaction between DNAH10, UCHL3, and PACRG in the context of manchette function.
Main Results:
- DNAH10 deficiency results in abnormal sperm head and flagella morphology.
- DNAH10 dysfunction impairs manchette function and leads to aberrant axonemal protein localization.
- DNAH10 acts as a bridge, enhancing the UCHL3-PACRG complex interaction crucial for manchette function.
Conclusions:
- DNAH10 plays a vital role in intra-manchette transport, essential for normal sperm development.
- Understanding DNAH10's function provides insights for genetic diagnosis and prognosis in male infertility patients.
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