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Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
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Tailored Metal-Organic Framework-Based Enzyme Hybrids: Immobilization Strategies, Improved Performance, and
Xiang Xu1,2, Jiacheng Wan2, Jun Sun2
1State Key Laboratory of Microbial Technology School of Food Science and Pharmaceutical Engineering Nanjing Normal University Nanjing 210023 China.
Small Science
|October 8, 2025
Summary
Metal-organic frameworks (MOFs) offer advanced solutions for immobilizing fragile natural enzymes. Precise MOF design enhances enzyme stability and catalytic efficiency for diverse applications.
Area of Science:
- Materials Science
- Biochemistry
- Nanotechnology
Background:
- Natural enzymes exhibit high catalytic efficiency and selectivity but suffer from poor stability.
- Metal-organic frameworks (MOFs) provide tunable structures ideal for enzyme immobilization.
- Current MOF-enzyme immobilization methods face challenges in balancing enzyme protection and accessibility.
Purpose of the Study:
- To review MOF-based enzyme immobilization strategies.
- To explore precise MOF design principles for overcoming limitations.
- To highlight applications of MOF-enzyme hybrids.
Main Methods:
- Categorization of immobilization strategies based on enzyme location (surface attachment, pore infiltration, encapsulation).
- Classification of MOF design strategies (microenvironment modulation, pore engineering, morphology tuning, defect engineering).
- Analysis of structure-property relationships in MOF-enzyme systems.
Main Results:
- Deeper enzyme embedding in MOFs enhances protection but can impede mass transfer.
- Precise MOF design significantly optimizes the enzymatic microenvironment and loading efficacy.
- MOF-enzyme hybrids demonstrate improved stability, catalytic efficiency, and mass transfer.
Conclusions:
- Advanced MOF design is crucial for enhancing enzyme performance and stability.
- MOF-enzyme hybrids show significant potential in biosensing, biocatalysis, drug delivery, and cancer therapy.
- Further exploration of MOFs can unlock broader applications for enzyme technology.
