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Updated: Jan 15, 2026

Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells
Published on: April 2, 2021
Saccharomyces cerevesiae, Candida spp. and Cryptococcus neoformans β-CAs
Anna Di Fiore1, Martina Buonanno1, Davide Esposito1
1Istituto di Biostrutture e Bioimmagini, CNR, Napoli, Italy.
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Carbonic anhydrases are ubiquitous metalloenzymes which catalyze the CO2 hydration to bicarbonate and proton. β-Carbonic Anhydrases from fungi, such as Saccharomyces cerevisiae, Candida spp. and Cryptococcus neoformans, have been widely investigated as potential targets for antifungal therapies. In this chapter, we provide a comprehensive overview on their properties highlighting their role as CO₂-sensing enzymes. We survey functional, biochemical, structural, and kinetic features, summarize inhibition and activation studies, and review in vitro experiments. Taken together, these data underscore fungal β‑carbonic anhydrases as promising potential targets for the development of new antifungal strategies.
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