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Updated: Aug 3, 2026

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
Published on: May 22, 2017
Assessing Perturbations in Lysosome Health by Flow Cytometry in iPSC-Derived Human Neuron Cultures
Kirstin O McDonald1, Stephanie M Hughes2, Indranil Basak3
1Department of Biochemistry, Faculty of Biomedical Sciences, Brain Health Research Centre, University of Otago, Dunedin, New Zealand.
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Fluorescent molecular probes have frequently been used to monitor lysosomal health, localization, abundance, and movement through the detection of acidic organelles and lysosomal enzyme activity. Flow cytometry technology provides rapid and accurate analysis of single cells (neurons) or particles (lysosomes) in suspension through laser detection. Herein, we describe how to detect lysosomes via LysoTracker™ and Magic Red® Cathepsin Activity assays in iPSC-derived human neuron cultures by flow cytometry.

