Related Experiment Video
Updated: Jan 15, 2026
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
Published on: May 22, 2017
Assessment of Lysosome Trafficking in iPSC-Derived Neurons Utilizing Time-Lapse Videos and TrackMate ImageJ Plugin
Oluwatobi N Eboda1, Indranil Basak2, Stephanie M Hughes3
1Department of Biochemistry, Brain Health Research Centre, University of Otago, Dunedin, New Zealand.
None:
In order to maintain cell homeostasis, lysosomes must be trafficked throughout the cell and associated with different compartments for the delivery and breakdown of macromolecules. This process is especially important in polar cells, such as neurons, that require the trafficking of lysosomes to distal locations to perform their functions. Here, we employed LysoTracker DND-99, a live-cell fluorometric dye that stains acidic organelles, to label lysosomes prior to time-lapse imaging on a Nikon A1 confocal microscope and subsequent assessment of total distance travelled using the TrackMate ImageJ plugin.
More Related Videos
10:38Author Spotlight: Understanding the Impact of Pathological Proteins on Axonal Transport in Neurodegenerative Diseases
Published on: December 22, 2023
13:13Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
Published on: March 19, 2021