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Peptide Identification Using Tandem Mass Spectrometry

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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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High-Contrast Aptamer-Based Merocyanine Displacement Assays for Sensitive Small Molecule Detection.

Thinh Nguyen1, Obtin Alkhamis1, Adara Bacon1

  • 1Department of Chemistry, North Carolina State University, 2620 Yarbrough Drive, Raleigh, North Carolina 27607, United States.

ACS Sensors
|October 13, 2025
PubMed
Summary
This summary is machine-generated.

A novel merocyanine dye enhances aptamer-based colorimetric assays for sensitive small-molecule detection. This dye offers improved color contrast and sensitivity compared to traditional methods, enabling on-site diagnostics.

Keywords:
aptamerblocking agentcocainemerocyaninesensorsolvatochromism

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Area of Science:

  • Chemical sensing
  • Biomolecular detection
  • Nanomaterials

Background:

  • Aptamer-based colorimetric assays are useful for on-site detection.
  • Current assays have limitations in sensitivity, color contrast, and spectral overlap.

Purpose of the Study:

  • To develop a novel indicator for highly sensitive small-molecule detection in dye-displacement assays.
  • To overcome the limitations of existing colorimetric aptamer-based sensing methods.

Main Methods:

  • Introduction of a solvatochromic coumarin-based merocyanine dye (X-19539-107).
  • Utilizing dye-displacement assays with diverse aptamers for small-molecule detection.
  • Spectroscopic analysis of dye monomers and aggregates.

Main Results:

  • The merocyanine dye X-19539-107 exhibits a large spectral shift (up to 180 nm) between H-aggregates and aptamer-bound monomers.
  • Achieved unprecedented target-induced dye displacement efficiency and sensitivity.
  • Demonstrated cocaine detection in saliva at toxicologically relevant levels.

Conclusions:

  • The merocyanine dye X-19539-107 is a powerful alternative to cyanine dyes for colorimetric aptamer-based sensing.
  • This novel dye enables highly sensitive and specific detection of small molecules.
  • The assay shows significant potential for real-world diagnostic applications.