Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Targeting Dental Pulp Stem Cell Senescence: A Potential Strategy for Improving Regenerative Endodontic Procedures.

Bioengineering (Basel, Switzerland)·2026

Same author

Cancer-Associated Alterations In O-GalNAc Glycosylation.

Glycobiology·2026

Same author

Mapping ST3GAL transferase specificities in the glycosylation landscape of N/TERT-1 keratinocytes using Glycogenomics and mass spectrometry.

Glycobiology·2026

Same author

Metabolic parameters as a predictor of relapse in patients with schizophrenia undergoing tapering of antipsychotics.

Journal of psychiatric research·2026

Same author

EatA mediated degradation of intestinal mucus is species-specific and driven by MUC2 structural features.

Nature communications·2025

Same author

Motivations for and Experiences With Antipsychotic Tapering Among Patients With Schizophrenia Seeking Guided Dose Reduction.

Psychiatric services (Washington, D.C.)·2024

Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 15, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Multiplexed Detection of Autoantibodies to Glycopeptides Using Microarray.

Johannes W Pedersen¹, Alexander Nøstdal¹, Sally Dabelsteen^1,2

¹Department of Cellular and Molecular Medicine, Copenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|October 14, 2025

Summary

This summary is machine-generated.

This study introduces a novel method for creating disease-specific O-glycopeptides using enzymes. These O-glycopeptides are then used with protein microarray technology to monitor antibody responses in patient serum for disease diagnosis.

Keywords:

Autoantibodies GalNAc Glycopeptide microarray Glycosylation Posttranslational modification Serum biomarkers Sialylation

More Related Videos

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Published on: May 29, 2020

Related Experiment Videos

Last Updated: Jan 15, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Published on: May 29, 2020

Area of Science:

Biochemistry
Immunology
Biotechnology

Background:

Protein microarrays are sensitive tools for detecting antibodies in serum.
Monitoring antibody titers is crucial for diagnosing infectious diseases, allergies, autoimmune diseases, and cancer.

Purpose of the Study:

To present a detailed protocol for the enzymatic generation of disease-specific O-glycopeptides.
To demonstrate the use of these O-glycopeptides in monitoring antibody responses via microarray technology.

Main Methods:

Enzymatic synthesis of O-glycopeptides specific to diseases.
Utilizing protein microarray technology for antibody detection in patient serum.

Main Results:

Successful generation of disease-specific O-glycopeptides.
Demonstrated ability to monitor antibody responses to these glycopeptides in serum.

Conclusions:

The presented protocol offers a novel approach for disease diagnosis.
Enzymatic O-glycopeptide generation combined with microarray technology is effective for monitoring antibody responses.