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Related Experiment Video

Updated: Jan 15, 2026

Killer Artificial Antigen Presenting Cells KaAPC for Efficient In Vitro Depletion of Human Antigen-specific T Cells
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Depleting Autoreactive B‑Cells Using Targeted Photodynamic Therapy.

Kevin R Venrooij1, Theodoros Ioannis Papdimitriou2, Daphne N Dorst1,2

  • 1Institute of Molecules and Materials, Synthetic Organic Chemistry, Radboud University, Heyendaalseweg 135, Nijmegen 6525 AJ, The Netherlands.

ACS Pharmacology & Translational Science
|October 16, 2025
PubMed
Summary

Targeted photodynamic therapy (tPDT) selectively eliminates autoreactive B cells using a novel construct. This approach spares healthy B cells, offering a promising treatment for autoimmune diseases like Rheumatoid Arthritis (RA).

Keywords:
antigen-selective B-cell targetingautoimmune diseasemultivalent antigensphotosensitizertargeted photodynamic therapy

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Area of Science:

  • Immunology
  • Biotechnology
  • Photomedicine

Background:

  • Autoimmune diseases like Rheumatoid Arthritis (RA) involve autoreactive B cells driving pathology.
  • Current B cell-depleting therapies lack specificity, causing broad immune deficiency.

Purpose of the Study:

  • To develop a targeted therapy to selectively eliminate autoreactive B cells.
  • To investigate the efficacy of targeted photodynamic therapy (tPDT) for this purpose.

Main Methods:

  • Designed a construct with a dimeric peptidic antigen (diCCP4) targeting a specific autoreactive B cell receptor (BCR).
  • Incorporated the photosensitizer IRDye700DX into the construct.
  • Tested the construct on a modified Ramos B-cell line expressing the target BCR.
  • Evaluated cell viability after light exposure in a 3D coculture with peripheral blood mononuclear cells (PBMCs).

Main Results:

  • The tPDT construct selectively eliminated modified Ramos B cells expressing the autoreactive BCR upon light activation.
  • The construct showed no cytotoxicity to cells lacking the target BCR.
  • Coculture experiments demonstrated minimal impact on untargeted cells.

Conclusions:

  • Targeted photodynamic therapy (tPDT) effectively and selectively eliminates autoreactive B cells.
  • This approach holds potential for treating autoimmune diseases like RA with reduced side effects.
  • Further research is warranted to translate this therapy to clinical applications.