Rapid Multianalyte Quantification of Brain Chemistry Using Si-Nanoribbon Bio-Field-Effect Transistor Sensors.

Area of Science:

• Neuroscience
• Biomedical Engineering
• Materials Science

Background:

• Continuous monitoring of neurochemicals like gamma-aminobutyric acid (GABA), lactate, and glutamate is crucial for understanding neurological disorders.
• Existing semiconductor field-effect transistor (FET)-based sensors have limitations in real-time detection and surface functionalization.

Purpose of the Study:

• To develop a complementary metal-oxide-semiconductor (CMOS)-based FET nanowire device for rapid, real-time, and simultaneous biosensing of GABA, lactate, and glutamate.
• To engineer the sensor surface for selective detection in buffered solutions and complex biological matrices.

Main Methods:

• Utilized a CMOS-based FET nanowire (nanoribbon) platform.
• Employed aptamer bioreceptors for lactate and glutamate, and monoclonal antibodies for GABA.
• Functionalized the sensor surface for selective, label-free detection.

Main Results:

• Achieved rapid quantification of neurochemicals within 10 minutes.
• Demonstrated highly selective, label-free detection using aptamers and antibodies.
• Reported limits of detection (LOD) as low as 80 fM for GABA, 58 fM for glutamate, and 182 fM for lactate.

Conclusions:

• The developed nanosensing platform enables continuous neurochemical monitoring.
• This technology shows significant potential for improving diagnostic accuracy and therapeutic efficacy in neurological care.