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A PSiO2/ZnO aptamer microarray chips based on "quenching-restoration" fluorescence strategy for multiple mycotoxin
Jiayu Chen1, Boxiang Yao1, Runzhong Yu2
1College of Food Science, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing, 163319, PR China.
Talanta
|October 20, 2025
Summary
A new aptamer microarray chip using PSiO2/ZnO nanostructures enables sensitive, high-throughput detection of multiple mycotoxins in cereals. This fluorescence-based method offers accurate quantification for improved food safety.
Area of Science:
- * Nanomaterials Science
- * Analytical Chemistry
- * Food Science
Background:
- * Mycotoxins in cereals pose significant risks to human health due to co-contamination and co-exposure.
- * Existing detection methods may lack the sensitivity and high-throughput capabilities required for comprehensive food safety analysis.
- * Advanced nanostructured materials offer potential for enhanced signal amplification and improved detection platforms.
Purpose of the Study:
- * To develop and evaluate a novel PSiO2/ZnO aptamer microarray chip for the simultaneous detection of multiple mycotoxins in cereals.
- * To establish a sensitive and specific fluorescence-based detection strategy for mycotoxin analysis.
- * To assess the chip's performance in terms of accuracy, stability, and applicability to real-world food samples.
Main Methods:
- * Fabrication of a PSiO2/ZnO composite nanostructured substrate for a microarray chip.
- * Immobilization of aptamers and complementary DNA strands to form a double-stranded DNA structure on the microarray.
- * Utilization of a fluorescence resonance energy transfer (FRET) based "quenching-restoration" strategy for signal detection.
- * Mycotoxin detection via specific binding to aptamers, leading to displacement of complementary strands and fluorescence recovery.
- * Integration with an improved QuEChERS method for sample preparation.
Main Results:
- * The PSiO2/ZnO aptamer microarray chip demonstrated high sensitivity for simultaneous detection of Ochratoxin A (OTA), Deoxynivalenol (DON), and Zearalenone (ZEN) in cereals.
- * Limit of detection (LOD) values were as low as 0.0137 ng/kg for OTA, 0.0185 ng/kg for DON, and 0.2730 ng/kg for ZEN.
- * The chip, combined with the QuEChERS method, showed good accuracy and stability for detecting mycotoxins in various cereal matrices.
- * The fluorescence "quenching-restoration" strategy enabled reliable qualitative and quantitative analysis.
Conclusions:
- * The developed PSiO2/ZnO aptamer microarray chip provides a sensitive, specific, and high-throughput platform for detecting multiple mycotoxins in cereals.
- * The "quenching-restoration" fluorescence strategy is effective for quantitative analysis of mycotoxins.
- * The method shows promise for application in routine food safety monitoring and the detection of other small molecules.
