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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jan 14, 2026

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Published on: May 2, 2016

Developing gold nanoparticles decorated with carbon-dots for multiplexed cellular imaging.

Tuğçe Kavuranpala¹, Iroda Saydullaeva^1,2, Serdar Ozcelik¹

¹Department of Chemistry, İzmir Institute of Technology, Urla-İzmir 35430, Türkiye.

|October 20, 2025

Summary

Researchers developed a new hybrid nanomaterial, gold nanoparticle decorated with carbon dots (AuNP@C-dot), for advanced cellular imaging. This dual-modal imaging platform enhances detection and monitoring of cell health and disease.

Keywords:

bioimaging cancer therapy carbon dots conjugation diagnosis gold nanoparticles

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Area of Science:

Nanomaterials Science
Biomedical Imaging
Cell Biology

Background:

Multiplexed imaging requires versatile platforms for simultaneous detection.
Gold nanoparticles (AuNPs) offer scattering signals, while carbon dots (C-dots) provide fluorescence.
Developing hybrid nanomaterials can combine distinct properties for enhanced functionality.

Purpose of the Study:

To create a novel hybrid nanomaterial, AuNP@C-dot, for multiplexed cellular imaging.
To characterize the structure and optical properties of the AuNP@C-dot hybrid.
To evaluate the in vitro performance and biocompatibility of AuNP@C-dots for cellular applications.

Main Methods:

Synthesis and characterization of AuNP@C-dot using FTIR, XPS, HRTEM, UV-Vis, and fluorescence spectroscopy.
In vitro studies on human lung cell lines (A549, BEAS-2B) for cellular uptake and localization.
Cell viability assays (colorimetric and colony formation) to assess biocompatibility.

Main Results:

Successful conjugation of C-dots to AuNPs confirmed via structural and spectral analyses.
Doubled fluorescence intensity of C-dots upon conjugation to AuNPs.
Demonstrated simultaneous multicellular imaging using fluorescence and scattering signals, with efficient cellular uptake and nuclear translocation observed in vitro.
AuNP@C-dots showed good biocompatibility at relevant concentrations.

Conclusions:

AuNP@C-dot hybrid nanomaterials offer a versatile platform for dual-modal multiplexed imaging.
The system exhibits efficient cellular uptake and biocompatibility, with potential applications in disease monitoring and targeted drug delivery.
This multifunctional tool holds promise for next-generation cellular analysis, diagnostics, and therapeutic strategies.