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A Method to Include Animal Fibre in Dietary Fibre Analysis.

Sylvie Marie-Thérèse Jean D'Hooghe1, Arturo Muñoz Saravia1, An Cools1

  • 1Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

Journal of Animal Physiology and Animal Nutrition
|October 21, 2025
PubMed
Summary

A new method accurately quantifies animal fibre in animal nutrition research. This adapted filter bag technique measures animal fibre, crucial for understanding carnivore and omnivore diets.

Keywords:
amylase‐treated neutral detergent fibreanimal fibregravimetricplant fibretotal dietary fibre

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Area of Science:

  • Animal nutrition science
  • Analytical chemistry
  • Digestive physiology

Background:

  • Animal fibre, from prey tissues like fur and bones, impacts carnivore health.
  • Accurate quantification of animal fibre is vital for nutrition research in carnivores and omnivores.
  • Existing fibre analysis methods are designed for plant fibre and cannot quantify animal fibre.

Purpose of the Study:

  • To adapt and validate a method for quantifying animal fibre.
  • To compare the new method with existing total dietary fibre (TDF) analysis.
  • To assess the method's efficacy across diverse sample types.

Main Methods:

  • Adapted the filter bag technique of the amylase-treated neutral detergent fibre method.
  • Incorporated a protease step to remove digestible protein.
  • Omitted sodium sulphite to preserve keratinaceous residues and used specific pre-extraction steps for fat and protein.

Main Results:

  • The adapted method, termed amylase-treated neutral detergent residue including animal fibre (aNDRa), successfully quantified animal fibre.
  • Traditional total dietary fibre (TDF) analysis yielded inaccurate and highly variable results for animal-based samples.
  • The aNDRa method demonstrated capability in quantifying both animal and plant fibre in various complex samples.

Conclusions:

  • The developed aNDRa method provides accurate quantification of animal fibre.
  • This advancement is critical for future research in animal nutrition, particularly for carnivores and omnivores.
  • The aNDRa method offers a reliable alternative to inaccurate TDF analyses for animal-based matrices.