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Updated: Jan 14, 2026
Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates
Published on: February 17, 2023
Enterococcus infantis sp. nov., isolated from the faeces of an infant with atopic dermatitis
Chin-Kan Chan1,2, Li-Jyun Tsai1, Misa Otoguro3
1Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd., Gui-Shan Township, Taoyuan County 333, Taiwan, ROC.
Insights
A novel Gram-positive bacterium, Enterococcus infantis sp. nov., was identified from an infant with atopic dermatitis. This discovery expands the understanding of the Enterococcus genus in human health.
Area of Science:
- Microbiology
- Bacteriology
- Genomics
Background:
- A novel Gram-positive, coccal bacterium, strain AD013-P3T, was isolated from a 10-month-old infant diagnosed with atopic dermatitis (AD).
- The isolation occurred from a stool specimen, suggesting a potential role in the infant gut microbiome.
Purpose of the Study:
- To characterize and classify the novel bacterial isolate AD013-P3T.
- To determine if the isolate represents a new species within the genus *Enterococcus*.
Main Methods:
- Phylogenetic analysis using 16S rRNA gene sequences.
- Genomic comparison via average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH).
- Determination of the G+C content of the genomic DNA.
Main Results:
- Phylogenetic analysis indicated the closest relatives were *Enterococcus diestrammenae* (98.7%) and *Enterococcus asini* (97.8%).
- Genomic analyses (ANI 75.4-80.4%, dDDH 22.1%) distinguished strain AD013-P3T from *E. diestrammenae* JCM 18359T.
- The G+C content was determined to be 43.5 mol%.
Conclusions:
- Strain AD013-P3T is phylogenetically and phenotypically distinct from known *Enterococcus* species.
- The data support the classification of strain AD013-P3T as a novel species, *Enterococcus infantis* sp. nov.
- The type strain is AD013-P3T (=NBRC 117136T=BCRC 81462T).
Abstract:
A Gram-positive, coccal-shaped organism, designated AD013-P3T, was isolated from the stool specimen of a 10-month-old male infant with atopic dermatitis (AD). The phylogenetic analysis based on 16S rRNA gene sequences showed that strain AD013-P3T was most closely related to Enterococcus diestrammenae (98.7%) and Enterococcus asini (97.8%). Genomic comparisons using pairwise average nucleotide identity and digital DNA-DNA hybridization analyses between strain AD013-P3T and E. diestrammenae JCM 18359T resulted in values of 75.4-80.4% and 22.1%, respectively. The G+C content of the genomic DNA of strain AD013-P3T was 43.5 mol%. Strain AD013-P3T is distinguishable from other established Enterococcus species both phylogenetically and phenotypically. Based on the data presented, strain AD013-P3T represents a novel species of the genus Enterococcus, for which the name Enterococcus infantis sp. nov. is proposed. The type strain is AD013-P3T (=NBRC 117136T=BCRC 81462T).
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