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Preparation of Cryptosporidium for Whole-Genome Sequencing
Guy Robinson1, Rachel M Chalmers2
1Cryptosporidium Reference Unit, Public Health Wales Microbiology and Health Protection, Singleton Hospital, Swansea, Wales, UK. Guy.Robinson@wales.nhs.uk.
None:
The application of whole-genome sequencing (WGS) to Cryptosporidium has been challenged by the relatively small numbers of oocysts present in feces and lack of suitable culture systems to generate sufficient DNA that is required. Here, we provide a method for the preparation of sufficient, high-quality Cryptosporidium DNA for WGS from feces, incorporating parasite purification stages and an optional whole-genome amplification step.
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