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A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

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Dual-Locked Small Molecule Fluorescent Probe for Precise Disease Imaging.

Xinru An¹, Pan Liang¹, Yongning Bian¹

¹Department of Chemistry, Beijing University of Technology, Beijing, 100124, P. R. China.

Chemistry, an Asian Journal

|October 28, 2025

Summary

Dual-locked fluorescent probes offer enhanced specificity for biological imaging by detecting two biomarkers simultaneously. This approach minimizes false positives, improving accuracy in disease diagnosis and therapy.

Keywords:

Biomarker Dual‐locked fluorescent probe Precise diagnosis Small molecule probe

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Area of Science:

Biomedical Imaging
Molecular Biology
Chemical Biology

Background:

Fluorescence imaging is crucial for noninvasive visualization of biomolecules in living systems.
Single-locked probes improve sensitivity but suffer from false positives due to nontarget biomarkers.
Dual-locked probes enhance specificity by detecting two biomarkers simultaneously.

Purpose of the Study:

To review recent advancements in dual-locked fluorescent probes for biological imaging.
To highlight diverse chemical structures and design strategies for these probes.
To discuss challenges and future directions for their application.

Main Methods:

Literature review of dual-locked fluorescent probe development.
Analysis of probe design strategies and chemical structures.
Discussion of probe performance in complex biological systems.

Main Results:

Dual-locked probes significantly improve specificity compared to single-locked probes.
Various chemical structures and design strategies enable targeted detection.
These probes show promise for precise identification of biomolecular events.

Conclusions:

Dual-locked fluorescent probes represent a significant advancement in specific biological imaging.
Further research is needed to overcome challenges in complex biological systems.
Future directions include optimizing probe design for enhanced in vivo applications.