Related Experiment Video
Updated: Jan 13, 2026
09:19
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
Published on: February 15, 2021
5.4K
Functional Validation of ALDOA in Regulating Muscle Cell Fate: Based on In Vitro Proliferation, Apoptosis, and
Hongzhen Cao1, Jing Wang2, Yunzhou Wang3
1Shandong Provincial Key Laboratory for Livestock Germplasm Innovation & Utilization, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China.
Genes
|October 29, 2025
Summary
AldoA (aldolase A) gene expression in skeletal muscle inhibits cell growth and promotes apoptosis. Overexpression of AldoA suppresses proliferation and myogenic differentiation, revealing dual metabolic and transcriptional roles in muscle cells.
Area of Science:
- Molecular Biology
- Cell Biology
- Muscle Physiology
Background:
- Aldolase A (ALDOA) is a key glycolytic enzyme.
- Its role in skeletal muscle beyond metabolism is not fully understood.
- Investigating ALDOA's function in muscle cell proliferation, apoptosis, and differentiation is crucial.
Purpose of the Study:
- To systematically investigate ALDOA gene expression characteristics in skeletal muscle cells.
- To determine the effects of ALDOA on skeletal muscle cell proliferation, apoptosis, and differentiation.
- To elucidate the regulatory mechanisms of ALDOA in muscle development and regeneration.
Main Methods:
- Constructed an ALDOA overexpression vector.
- Transfected the vector into C2C12 cells and porcine skeletal muscle satellite cells.
- Analyzed gene expression, cell cycle, apoptosis, and differentiation markers.
Main Results:
- ALDOA showed highest expression in longissimus dorsi muscle and nuclear localization.
- ALDOA overexpression inhibited proliferation (G0/G1 arrest, decreased CDK2/Cyclin D1) and promoted apoptosis.
- ALDOA overexpression suppressed myogenic differentiation markers (MYOD, MYOG, MEF2C) despite upregulation during normal differentiation.
Conclusions:
- ALDOA acts as a multifunctional regulator in skeletal muscle cells.
- It negatively controls cell growth by inhibiting proliferation and promoting apoptosis.
- ALDOA impedes myogenic differentiation, indicating roles beyond glycolysis in muscle cellular processes.
