Related Experiment Video
Updated: Mar 29, 2026
07:51
Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
Published on: April 30, 2014
21.3K
PDGFD: A Dual-Function Regulator That Maintains Myoblast Pool and Fuels Myogenic Differentiation
Hongzhen Cao1, Jing Wang2, Yunzhou Wang3
1Shandong Provincial Key Laboratory for Livestock Germplasm Innovation & Utilization, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China.
Current Issues in Molecular Biology
|March 28, 2026
Summary
Platelet-derived growth factor D (PDGFD) maintains the myoblast pool and initiates muscle cell differentiation. This study reveals PDGFD
Area of Science:
- Muscle biology
- Cellular signaling
- Developmental biology
Background:
- The function of platelet-derived growth factor D (PDGFD) in mesenchymal cells is known, but its role in skeletal muscle generation is unclear.
- PDGFD is identified as a candidate gene for muscle and fat development in pigs.
Purpose of the Study:
- To elucidate the specific role of PDGFD in skeletal muscle generation and myogenesis.
- To investigate the regulatory mechanisms of PDGFD in myoblast proliferation, apoptosis, and differentiation.
Main Methods:
- Single-cell RNA sequencing of skeletal muscle from Jiangquan Black pigs.
- C2C12 cell line experiments involving PDGFD knockdown and overexpression.
- Analysis of myogenic differentiation markers (MyoD, MyoG) and signaling pathways (PI3K/Akt).
Main Results:
- PDGFD knockdown inhibited proliferation and promoted apoptosis in myoblasts; overexpression enhanced viability.
- PDGFD interference downregulated MyoD and MyoG, inhibiting myogenic differentiation.
- PDGFD expression peaks during mid-differentiation, suggesting temporal regulation, potentially via a pathway independent of PI3K/Akt.
Conclusions:
- PDGFD plays a dual role in myogenesis, acting as both a guardian of the myoblast pool and an initiator of differentiation.
- This study expands the known functions of PDGFD and provides novel insights into growth factor-mediated cell fate decisions in muscle development.
Related Concept Videos
02:23Master Transcription Regulators
8.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.0K
01:22iPS Cell Differentiation
3.3K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
3.3K
01:13Formation of Muscle Fibers from Myoblasts
7.0K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
7.0K