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The Next Dimension: Digital Holography for 3D Interferometric Scattering.

Jaime Ortega Arroyo1, Matz Liebel2

  • 1Nanophotonic Systems Laboratory, Department of Mechanical and Process Engineering, ETH-Zürich, 8092 Zürich, Switzerland.

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|October 30, 2025
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Summary

This study details digital holography methods for high-sensitivity interferometric scattering (iSCAT) nanosizing. The guidelines cover experimental setup, data processing, and applications for precise particle characterization.

Keywords:
digital holographyinterferometric scattering microscopylabel-free imagingnanosizingscatterometrysingle-particle tracking

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Area of Science:

  • Optics and Photonics
  • Nanotechnology
  • Biophysics

Background:

  • Interferometric scattering (iSCAT) microscopy is a powerful technique for label-free detection and sizing of nanoparticles.
  • Traditional iSCAT methods can be limited in resolution and dynamic range.
  • Digital holography offers a versatile interferometric approach with potential for enhanced nanosizing capabilities.

Purpose of the Study:

  • To provide comprehensive experimental guidelines for implementing digital holography with iSCAT for high-sensitivity nanosizing.
  • To highlight the advantages of off-axis digital holography over conventional iSCAT strategies.
  • To detail data processing techniques for advanced particle analysis.

Main Methods:

  • Utilizing off-axis digital holography for interferometric measurements.
  • Presenting detailed experimental setups and implementation strategies.
  • Describing digital data processing routines including refocusing, 3D particle tracking, and aberration correction.

Main Results:

  • Demonstration of digital holography's compatibility with iSCAT for nanosizing.
  • Identification of key experimental parameters for optimizing performance.
  • Successful application of digital postprocessing for enhanced particle characterization.

Conclusions:

  • Digital holography provides a versatile and advantageous platform for high-sensitivity iSCAT-based nanosizing.
  • The presented guidelines enable robust implementation and advanced analysis of nanoparticle data.
  • Future applications in particle characterization and beyond are promising.