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Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma
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High-resolution imaging mass cytometry to map subcellular structures.

Alina Bollhagen1,2,3, James Whipman1,2,3, Ricardo Coelho4

  • 1Department of Quantitative Biomedicine, University of Zurich, Zurich, Switzerland.

Nature Methods
|October 31, 2025
PubMed
Summary
This summary is machine-generated.

High-resolution imaging mass cytometry (HR-IMC) achieves submicrometer resolution, revealing previously undetectable subcellular structures. This advancement enables detailed analysis of cell biology and disease characteristics.

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Area of Science:

  • Biomedical Imaging
  • Cell Biology
  • Pathology

Background:

  • Imaging mass cytometry (IMC) is a key technology for analyzing cell phenotypes and tissue organization.
  • Current IMC resolution (1 µm) limits visualization of submicrometer intracellular structures.
  • There is a need for higher resolution imaging techniques in cell biology research.

Purpose of the Study:

  • To develop and validate a method for enhancing the spatial resolution of IMC.
  • To enable visualization of subcellular structures with IMC.
  • To apply enhanced IMC to study disease mechanisms at a finer resolution.

Main Methods:

  • Developed high-resolution IMC (HR-IMC) using an oversampling approach.
  • Implemented point-spread function-based deconvolution to improve image resolution.
  • Applied HR-IMC to analyze subcellular structures and chemotherapy-induced cellular changes.

Main Results:

  • Achieved IMC resolution below 350 nm, approaching light microscopy.
  • Successfully resolved subcellular structures like nuclear foci and mitochondrial networks.
  • Visualized chemotherapy-induced perturbations in patient-derived ovarian cancer cells.

Conclusions:

  • HR-IMC significantly enhances the capabilities of multiplexed imaging.
  • This technique allows for detailed analysis of intracellular features and disease characteristics.
  • HR-IMC opens new avenues for studying cell biology and pathology with unprecedented detail.