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Ultra-Rapid DNA Extraction Coupled with Loop-Mediated Isothermal Amplification for Point-of-Care Detection of

Silvia A Longhi1, Lady J García-Casares1, Arturo A Muñoz-Calderón1

  • 1Laboratorio de Biología Molecular de La Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"- Consejo Nacional de Investigaciones Científicas y Técnicas (INGEBI-CONICET), Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.

Methods in Molecular Biology (Clifton, N.J.)
|November 3, 2025
PubMed

Keywords:
Congenital Chagas diseaseDried blood spotLoop mediated isothermal amplificationMolecular diagnosisPoint-of-care detection

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...