Lyophilization Improved Efficiency in 2-in-1 Metabolite and Protein Extraction for Plant Multi-Omics

Area of Science:

• Plant Biology
• Molecular Biology
• Biochemistry

Background:

• Integrated omics enables systematic analysis of genes and molecules.
• Using the same sample for multi-omics data ensures consistency.
• Current methods may not efficiently handle simultaneous metabolite and protein extraction from plants.

Purpose of the Study:

• To develop an efficient method for co-extracting metabolites and proteins from single plant samples.
• To optimize sample preparation for plant integrative multi-omics studies.
• To improve throughput and data integration in plant system biology.

Main Methods:

• Developed a novel protocol for simultaneous metabolite and protein extraction from plant tissues.
• Compared protein extraction efficiency between fresh and lyophilized samples across various plant tissues and species.
• Investigated the impact of metabolite pre-extraction on subsequent protein extraction.

Main Results:

• The optimized method allows co-extraction of metabolites and proteins within 2 hours.
• Lyophilized plant samples demonstrated significantly higher protein extraction efficiency than fresh samples.
• Pre-extraction of metabolites simplified the protein extraction process, eliminating the need for precipitation steps.

Conclusions:

• This rapid, high-throughput method facilitates integrated omics analyses in plants.
• The use of lyophilized samples enhances protein recovery for multi-omics studies.
• The developed technique supports efficient sample preparation for plant system biology research.