Biocompatible Nanozyme Shell-Armed Probiotic with Inflammation Targeting and Scavenging Properties Enables Effective...

Area of Science:

• Biomaterials Science
• Microbiology
• Nanotechnology

Background:

• Probiotics offer therapeutic potential but require protection from harsh environments and targeted delivery.
• Developing biocompatible methods to enhance probiotic bioactivity and targeting is essential for effective treatment.

Purpose of the Study:

• To develop a novel nanozyme shell for probiotics that provides protection, targets inflammation, and scavenges reactive oxygen species (ROS).
• To evaluate the efficacy of nanozyme shell-armed probiotics in a mouse model of colitis.

Main Methods:

• Utilized metformin to create a polydopamine-based nanozyme shell (DMCe) on probiotics.
• Assessed the protective barrier function, ROS scavenging capacity, and anti-inflammatory effects of DMCe-probiotics.
• Investigated the impact on microbial community composition and short-chain fatty acid levels.
• Evaluated therapeutic outcomes in a mouse colitis model.

Main Results:

• The DMCe shell provided a robust barrier against digestive juices and antibiotics.
• DMCe synergistically scavenged ROS at colitis lesions, utilizing ROS to prolong inflammation targeting up to 7 days.
• Nanozyme shell-armed probiotics improved microbial composition and increased short-chain fatty acids.
• Significant reduction in inflammation and promotion of mucosal barrier healing observed in the colitis model.

Conclusions:

• Nanozyme shell-armed probiotics offer a promising strategy for treating colitis by integrating inflammation targeting, ROS scavenging, and microbiome remodeling.
• This biocompatible approach preserves probiotic bioactivity and enhances therapeutic outcomes without complex chemical modifications.