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Updated: Jan 12, 2026
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
Analysis of the main components of LSPN based on broadly targeted metabolomics technology
Feng Qin1,2, Yuyong Chen3, Dada Wang4
1Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Engineering Technology Research Center for Modern Animal Science and Novel Veterinary Pharmaceutic Development, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, Jiangsu, China.
Introduction:
This study aimed to (1) establish a broadly targeted metabolomics workflow to evaluate batch-to-batch variability in Panax notoginseng-derived Panax notoginseng stem/leaf-derived oral liquid (LSPN) preparations, (2) identify critical process-induced metabolite differences (e.g., saponin degradation products), and (3) correlate these differences with pharmacopoeial quality markers (e.g., Rg1/Rb1 ratios).
Methods:
Our hypothesis was that thermal extraction parameters would significantly alter saponin profiles, detectable via orthogonal metabolomic approaches (HPLC-MS/MS, PCA).
Results:
The results showed a strong positive correlation (pearson correlation coefficient >0.92) between triterpenoid saponins in LSPN-S and LSPN-C. Thirty-eight triterpenoid saponins were detected, of which 11 were up-regulated, 5 were down-regulated, and the other 22 had less than a two-fold difference in content. Ginsenoside Rb3 showed a modest but significant downregulation in LSPN-S (fold change = 0.45, VIP = 1.11) compared with LSPN-C.
Discussion:
Metabolomic analysis confirmed the suitability of the selected Panax notoginseng stems/leaves as raw materials, with comprehensive characterization of LSPN's primary components.
