Related Experiment Video

Updated: Jun 21, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

14.4K

Cas9-Leveraged Single-Molecule Characterization of Sparse Plasmid Vectors in Heterogenous DNA Samples

Carl Möller1, Luis Leal-Garza1, Emanuele Celauro2

  • 1Department of Life Sciences, Chalmers University of Technology, Gothenburg, SE, 41296, Sweden.

Applied Biochemistry and Biotechnology
|November 8, 2025
PubMed
Summary

No abstract available in PubMed .

Keywords:
Adeno associated virus (AAV)Cas9Circular DNAConcatemerizationDNA sizingEpisomal DNAFluorescence microscopySingle moleculeYOYO-1

More Related Videos

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
06:51

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

4.3K
Plasmid Stability Analysis with Open-Source Droplet Microfluidics
07:43

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Published on: December 27, 2024

1.0K

Related Experiment Videos

Last Updated: Jun 21, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

14.4K
Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
06:51

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

4.3K
Plasmid Stability Analysis with Open-Source Droplet Microfluidics
07:43

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Published on: December 27, 2024

1.0K

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

Articles linked to this work by shared authors, journal, and citation graph.

MYC modulates TOP2A diffusion to promote substrate detection and activity.

Nature communications·2026

Expanding the DNA damaging potential of artificial metallo-nucleases with click chemistry.

Nature communications·2026

DOGMA: de novo assembly of densely labelled optical DNA maps using a matrix profile approach.

PloS one·2025

Oligomerisation of Ku from Mycobacterium tuberculosis promotes DNA synapsis.

Nature communications·2025

Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR.

Nature communications·2025

Emergence of carbapenem-producing enterobacteriaceae (CPE) and other multidrug-resistant gram-negative bacteria in neonates at a tertiary-level NICU in Tanzania: a point prevalence study.

JAC-antimicrobial resistance·2025

m6A-Mediated Stabilization of S100A10 mRNA by IGF2BP3 Aggravates Environmental Cigarette Smoke-Induced Asthma in Children.

Applied biochemistry and biotechnology·2026

A Comprehensive Review of Recent Advances in Sustainable Synthesis of Bio-Based Furanic Compounds Via Chemical, Biological, Chemobiological and Other Combination Approach in Benign Systems.

Applied biochemistry and biotechnology·2026

Transaminases engineering and their cascade systems for natural product synthesis.

Applied biochemistry and biotechnology·2026

Exploring the Biocompatibility and Therapeutic Potential of Graphene Oxide-Based Nanomaterials in Modern Medicine. A Comprehensive Review.

Applied biochemistry and biotechnology·2026

Green Synthesis of Silver Nanoparticles from Cotoneaster lacteus: Experimental Characterization and Mechanistic Insights from Biological Assays, DFT and Molecular Docking Studies.

Applied biochemistry and biotechnology·2026

Electrochemical Quantification of Parathyroid Hormone Using an Aptamer-Functionalized Magnetic Nanoparticle System.

Applied biochemistry and biotechnology·2026

Evaluation of Antisense Oligonucleotide-Associated Neuronal Lysosomal Changes.

Nucleic acid therapeutics·2026

Computational investigation for exploring botanical ingredients as potential negative allosteric modulators targeting metabotropic glutamate receptor 5.

Research in pharmaceutical sciences·2026

Integrated analysis with iCM-SANS, SAXS and MD simulations for dynamics of multi-domain proteins.

Journal of applied crystallography·2026

Lacosamide Use After Traumatic Brain Injury: A Scoping Review.

Cureus·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us