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Bis-Pseudoindoxyls: A Compact Fluorophore with Red-Shifted Absorption and Fluorescence for Bioimaging Applications
Tomohiro Yazawa1, Masaya Nakajima2, Akiko Takaya1,3
1Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba 260-8675, Japan.
Abstract:
A new class of single benzene-based fluorophores, bis-pseudoindoxyls, was developed. The core scaffold 14CO25NH is constructed via a visible-light-induced radical cyclization. Subsequent N-alkylation produced pronounced bathochromic shifts. The optimized dye 14CO25Nallyl exhibited absorption and emission maxima at 621 and 687 nm, respectively, with a moderate quantum yield in CHCl3. These properties enable long-term live-cell imaging of lipid droplets in the red spectral region.
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